This peculiar switching process is also involved in other functions, such as increasing antibiotic tolerance and protecting the cell against reactive oxygen species (ROS), a family of potentially harmful free radicals. Interestingly, small sulphur-containing molecules called sulfides also affect this switching process. Bacteria seem to have evolved ways to sense hydrogen sulfide (H₂S) levels and adapt their metabolism accordingly. How exactly they do this, however, is not well understood.

To address this knowledge gap, a research team including Associate Professor Shinji Masuda from Tokyo Institute of Technology, Japan, recently focused on YgaV, a transcription factor found in the enteric bacteriumEscherichia colithat could add some pieces to the puzzle. In their latest paper, which was published inAntioxidants, the team explains that YgaV is evolutionarily related (and almost identical) to certain proteins previously discovered in other bacteria that respond to H₂S levels. This motivated them to decipher what the role of YgaV is and how it helpsE. colithrive when the chemical conditions around them change.

The researchers conducted a series of experiments in normal (or WT, for 'wild-type') and mutantE. colistrains, the latter of which had a non-functionalygaVgene. They exposed these cultures to different atmospheric conditions (oxygen-rich, oxygen-deficient, and H₂S-rich). Then, through transcriptome analysis, the team investigated which genes were expressed differently between the WT and mutant strains, thus highlighting the role of YgaV. They also exposed both strains of bacteria to several types of antibiotics and compared their effects. Further, they measured the cellular concentration of oxygen peroxide (H₂O₂), a type of ROS, and analyzed some of the structural details of how YgaV binds to sulphur atoms.

Overall, the results showed that YgaV is crucial for fine-tuning the expression of various anaerobic respiratory genes and managing the levels of ROS in response to external H₂s .值得注意的是,抗生素耐受性是不同的between strains, as Associate Professor Masuda remarks: "While the WT bacteria showed increased antibiotic tolerance under H₂S-atmospheric conditions, theygaVmutant did not. Additionally, antibiotic sensitivity was higher in the mutant than in the WT strain, both in the presence and absence of external H₂S."

These findings clarify some of the mechanisms by which various bacteria can regulate their internal chemical balance in response to changes in their surroundings. This knowledge could be used to identify potential drug targets to make pathogenic bacteria more susceptible to antibiotics. In turn, this would help in the treatment of infectious diseases. Most importantly, the link that exists between YgaV, antibiotic tolerance, and ROS levels can be studied in more detail to gain a better understanding of how antibiotic resistance works. "Further research on YgaV may contribute to solving the decade-old enigma of whether there is a relationship between ROS generation and antibiotic tolerance," notes Associate Professor Masuda.