WhileE. colibacteria are one of the most well-known causes of food poisoning, a wide variety ofE. colistrains exists, many of which are harmless, permanent residents of our intestines. However, the ingestion of harmful strains ofE. colion contaminated food can lead to severe illness, vomiting, and diarrhea.

"To develop the most effective preventive measures, we need a deep understanding of the source and living conditions of the bacteria," says Yoshitoshi Ogura, associate professor at Kyushu University's Department of Bacteriology, who led the research.

"Although cattle have long been thought to be a main source ofE. colithat cause food poisoning, why dangerous forms would keep appearing in cattle has been unclear."

Ogura's group, in collaboration with researchers across Japan and in France, Belgium, and the United States, set out to help answer this question by investigating the genetics ofE. colibacteria collected from cattle and humans in 21 countries spanning six continents.

"To date, there have been only a limited number of reports of the genome sequences ofE. colifrom cattle, so we needed to fill that gap," comments Yoko Arimizu, first author on the paper inGenome Researchannouncing the new results.

While the largest number of samples was from Japan, strains from other regions exhibited characteristics that were well distributed among those from Japan, indicating a good diversity of the set of samples.

Based on the genetic features of the bacteria, the researchers could generally separate the different strains ofE. coliinto two groups, with one primarily consisting of bacteria collected from humans and the other of those from cattle.

Applying the same analysis to clinically obtainedE. colithat are known to cause illness, the researchers found that most of the strains causing intestinal problems belonged to the group associated with cattle.

Furthermore, many of the samples from cattle exhibited features similar to those causing food poisoning, such as the production of Shiga toxin. While these features generally appear not to cause illness in cattle, their prevalence in the investigated samples suggests that such characteristics are beneficial for life in a cattle's intestine.

"As long as there is pressure to maintain or strengthen these illness-producing characteristics to better adapt to living in a cattle's intestine, new variants ofE. colithat cause food poisoning are likely to continue appearing," states Ogura.

The researchers speculate that these characteristics may helpE. coliprotect itself from bacteria-eating organisms present in cattle intestines, but more work is needed to identify the exact reason.