Formic acid is a carboxylic acid composed of one carbon. Formic acid was produced from CO₂by the chemical method. Recently, the C1 Gas Refinery R&D Center has successfully developed a biological process that produces formic acid from carbon monoxide for the first time. Formic acid is in a liquid state when at room temperature and atmospheric pressure. In addition, it is chemically stable and less toxic, thus, easy to store and transport. Therefore, it can be used as an alternative carbon source in the microbial fermentation process. In order to produce value-added chemicals using formic acid, a metabolic pathway that converts formic acid into cellular molecules composed of multiple carbons is required. However, a metabolic pathway that can efficiently convert formic acid into cellular molecules has not been developed. This acted as an obstacle for the production of value-added chemicals using formic acid

A research group of Ph.D. student Junho Bang and Distinguished Professor Sang Yup Lee of the Department of Chemical and Biomolecular Engineering at the Korea Advanced Institute of Science and Technology (KAIST) addressed this issue. This study, entitled "Assimilation of Formic Acid and CO₂by Engineered Escherichia coli Equipped with Reconstructed One-Carbon Assimilation Pathways," has been published online in theProceedings of the National Academy of Sciences (PNAS)on September 18.

There has been increasing interest in utilizing formic acid as an alternative carbon source for the production of value-added chemicals. This research reports the development of an engineeredE. colistrain that can convert formic acid and CO₂to pyruvate and produce cellular energy from formic acid through the reconstructed one-carbon pathways.

The metabolic pathway that efficiently converts formic acid and CO₂into pyruvate was constructed by the combined use of the tetrahydrofolate cycle and reverse glycine cleavage reaction. The tetrahydrofolate cycle was reconstructed by utilizing Methylobacterium extorquens formate-THF ligase, methenyl-THF cyclohydrolase, and methylene-THF dehydrogenase. The glycine cleavage reaction was reversed by knocking out the repressor gene (gcvR) and overexpressing the gcvTHP genes that encode enzymes related with the glycine cleavage reaction. Formic acid and CO₂conversion to pyruvate was increased via metabolic engineering of theE. colistrain equipped with the one-carbon assimilation pathway.

此外,为了减少葡萄糖consumption and increase formic acid consumption, Candida boidnii formate dehydrogenase was additionally introduced to construct a cellular energy producing pathway from formic acid. This reduces glucose consumption and increases formic acid consumption.

The reconstructed one-carbon pathways can supply cellular molecules and cellular energies from the formic acid and CO₂. Thus, the engineeredE. colistrain equipped with the formic acid and CO₂assimilation pathway and cellular energy producing pathway from formic acid showed cell growth from formic acid and CO₂without glucose. Cell growth was monitored and 13C isotope analysis was performed to confirmE. coligrowth from the formic acid and CO₂. It was found that the engineeredE. colistrain sustained cell growth from the formic acid and CO₂without glucose.

Professor Lee said, "To construct the C1-refinery system, a platform strain that can convert one-carbon materials to higher carbon materials needs to be developed. In this report, a one-carbon pathway that can efficiently convert formic acid and CO₂to pyruvate was developed and a cellular energy producing pathway from formic acid was introduced. This resulted in an engineeredE. colistrain that can efficiently utilize formic acid as a carbon source while glucose consumption was reduced. The reconstructed one-carbon pathways in this research will be useful for the construction of the C1-refinery system."