Study reveals 'bug wars' that take place in cystic fibrosis

The results provide insights into how bacterial pathogens wrestle each other for territory that could open avenues for new antibacterial treatments.

Studies of microbes from mouths, intestines, chronic wounds and chronic respiratory infections show that interactions between bacteria in these communities influence survival of the bugs and progression of disease. For example, infection with two bacterial species calledStaphylococcus aureus(S. aureus) andPseudomonas aeruginosa(P. aeruginosa) in the airways of cystic fibrosis patients is linked to decreased lung function and a shorter lifespan.

"One strategy to improve outcomes for people with cystic fibrosis carrying multiple infections is to block harmful interspecies interactions before they begin," explains lead author Dominique Limoli, Assistant Professor of Microbiology and Immunology at the University of Iowa, US. "We designed a system to visualise the early interactions between two microbes that cause infections in people with cystic fibrosis to follow their behaviour over time."

Limoli and her team used a co-culture system to grow both microbes together and then studied them using time-lapse microscopy to create videos of the bacteria's movements. They found that, on their own,P. aeruginosacells multiply and form groups that appear like 'rafts' under a microscope. By contrast, when grown along withS. aureus,P. aeruginosacells move as single cells and accelerate their movement in the direction of theS. aureuscolony. Studying the cells in more detail revealed that once the singleP. aeruginosacells reach theS. aureuscells, they enter the colony and dismantle it.

The researchers proposed thatP. aeruginosabehaves in this way becauseS. aureusproduces substances into the environment that trigger the change in movement ofP. aeruginosa. To prove this, they took the liquid thatS. aureushad been growing in and placed it in the bottom of a petri dish, before using it to growP. aeruginosa. In this environment,P. aeruginosawas much more mobile -- moving across a greater surface area than on the normal petri dish.

So, does this hold true for other types of bacteria? Of three pathogens commonly found in patients with cystic fibrosis, the team found one that enhanced the movement ofP. aeruginosa. However, when they looked at a broader group of bacteria, including Salmonella, Escherichia coli and Bacillus subtilis, they found a significant increase in the movement ofP. aeruginosain the presence of these communities.

"By acquiring a fundamental understanding of how bacteria sense and respond to life with each other, we move closer to learning how to rationally manipulate these behaviors during infection and in the environment," says Limoli. "For cystic fibrosis patients, this may mean preventingP. aeruginosaandS. aureusphysical interactions. In other instances, we might bring together bacterial species that synergise to produce a beneficial compound."