'Tug of war' between cells – When crucial connections are missing

The researchers investigated the connections between the cells using a range of biophysical techniques. Employing video microscopy and automated analyses, they examined the properties of thousands of living cells in motion for over twenty hours. Their investigations included observing the size, shape, and mobility of the cells. Specific proteins were also stained to find out what happens at a molecular level when the "tight junction" connections are lost.

If these special connections are missing, neighbouring cells can no longer couple with each other as they usually would. The individual cells start to contract very strongly and a kind of "tug of war" develops between the cells. Lead author Professor Andreas Janshoff, University of Göttingen, says: "Some cells win and contract to tiny sizes, while other cells lose and get stretched apart. The loser cells even start dividing more in the process, creating more and more small winner cells." The contracted cells hardly move at all, so that the whole tissue behaves as if it is frozen. If, on the other hand, these special connections between the cells are present, the cell layer remains very mobile, similar to liquid water. For example, in healthy tissue, wounds can heal efficiently in this way, while the epithelial cells simultaneously maintain their barrier function.

“我们惊讶地发现这样一个机制creates an extremely unequal tug of war between cells that are essentially identical," says first author Mark Skamrahl at the University of Göttingen. "The negative influence of this tug of war on cell movement is another new finding about how cell mechanics can critically determine biological functions."

In previous research, only the mechanical role of a type of connection known as "adherens junctions" in collective cell movement was known. The importance of "tight junctions" is not only essential to the understanding of cell movement, it could also contribute to the understanding of cancer.