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Brain organoids mimic head size changes associated with type of autism

Date:
August 25, 2021
Source:
加州大学-圣迪ego
Summary:
Stem cell models derived from people with specific genomic variation recapitulate aspects of their autism spectrum disorder, providing a valuable model to study the condition and look for therapeutic interventions.
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FULL STORY

Variations in the 16p11.2 region of the genome are associated with autism spectrum disorder. While people with genetic deletions in this region have larger heads (macrocephaly) and people with genetic duplications have smaller heads (microcephaly), both variation types affect brain development and function.

圣udy the effects of these variations and search for ways to minimize their impact, University of California San Diego School of Medicine researchers are using brain organoids -- tiny, 3D cellular models generated in the lab from people with 16p11.2 variations.

The organoids, described in a paper publishing August 25, 2021 inMolecular Psychiatry, mimicked the differences in brain size seen in people. They also revealed new information about the molecular mechanisms that malfunction when the 16p11.2 region of the genome is disrupted, providing new opportunities for potential therapeutic intervention.

"Because our organoids recapitulate the head size of the patients, that tells us this can be a useful model," said senior author Lilia Iakoucheva, PhD, associate professor of psychiatry at UC San Diego School of Medicine. "And we need better models to study autism spectrum disorder, especially during fetal development."

Iakoucheva led the study with Alysson Muotri, PhD, professor of pediatrics and cellular and molecular medicine at UC San Diego School of Medicine.

The brain organoids were created using induced pluripotent stem cells derived from people who have 16p11.2 genomic variations -- three people with deletions, three with duplications and three non-variant controls. Researchers obtained a skin sample from each person, gave the skin cells a molecular cocktail that converted them to stem cells, then treated the stem cells in a way that coaxed them into becoming brain cells, preserving each patient's unique genetic background.

The organoids revealed that RhoA -- a protein that plays a big part in many basic cellular functions, such as development and movement -- is more active in both 16p11.2-deleted and 16p11.2-duplicated organoids than it is in organoids without these variations. Over-active RhoA led to a slowdown in neuronal migration, the process by which brain cells get to where they need to be for normal fetal development and function in adulthood.

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When the team inhibited RhoA in the autism-like organoids, neuronal migration was restored to levels seen in the control organoids.

"Our work opens the possibility to therapeutically manipulate the RhoA pathway," said Muotri, who is also director of the UC San Diego Stem Cell Program and a member of the Sanford Consortium for Regenerative Medicine. "The same pathway may be also damaged in other individuals with autism spectrum disorder who have macrocephaly or microcephaly. Considering this, we can potentially help millions of patients."

Organoids aren't perfect reproductions of the brain. They lack connections to other organ systems, such as blood vessels, and so don't encapsulate full human biology. In addition, therapeutics tested on brain organoids are added directly. They don't need to get across the blood-brain barrier, specialized blood vessels that keep the brain largely free of microbes and toxins.

The team plans to further test RhoA inhibitors in a mouse model with 16p11.2 variations or over-active RhoA for their ability to reverse defects associated with autism spectrum disorder.

Co-authors include: Jorge Urresti, Pan Zhang, Patricia Moran-Losada, Priscilla D. Negraes, Cleber A. Trujillo, Danny Antaki, Megha Amar, Kevin Chau, Akula Bala Pramod, Leon Tejwani, Sarah Romero, and Jonathan Sebat, all at UC San Diego; Nam-Kyung Yu, Jolene Diedrich, and John R. Yates III, Scripps Research.

Story Source:

Materialsprovided by加州大学-圣迪ego. Original written by Heather Buschman, PhD.Note: Content may be edited for style and length.


Journal Reference:

  1. Jorge Urresti, Pan Zhang, Patricia Moran-Losada, Nam-Kyung Yu, Priscilla D. Negraes, Cleber A. Trujillo, Danny Antaki, Megha Amar, Kevin Chau, Akula Bala Pramod, Jolene Diedrich, Leon Tejwani, Sarah Romero, Jonathan Sebat, John R. Yates III, Alysson R. Muotri, Lilia M. Iakoucheva.Cortical organoids model early brain development disrupted by 16p11.2 copy number variants in autism.Molecular Psychiatry, 2021; DOI:10.1038/s41380-021-01243-6

Cite This Page:

加州大学-圣迪ego. "Brain organoids mimic head size changes associated with type of autism." ScienceDaily. ScienceDaily, 25 August 2021. /releases/2021/08/210825211212. htm>.
加州大学-圣迪ego. (2021, August 25). Brain organoids mimic head size changes associated with type of autism.ScienceDaily. Retrieved September 6, 2023 from www.koonmotors.com/releases/2021/08/210825211212. htm
加州大学-圣迪ego. "Brain organoids mimic head size changes associated with type of autism." ScienceDaily. www.koonmotors.com/releases/2021/08/210825211212. htm(accessed September 6, 2023).

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