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Gene variant linked to sleep problems in autism

Date:
April 29, 2019
Source:
Washington State University
Summary:
研究人员发现,睡眠问题在patients with autism spectrum disorder may be linked to a mutation in the gene SHANK3 that in turn regulates the genes of the body's 24-hour day and night cycle. Their study showed that people who were missing the SHANK3 gene and mice that lacked part of the gene had difficulty falling asleep.
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FULL STORY

Up to 80 percent of children with autism spectrum disorder (ASD) experience sleep problems. The source of these problems has been as much of a mystery as the exact causes of ASD, which scientists are still working to unravel. A new study led by a team of neuroscientists at Washington State University has brought scientists closer to identifying the causes of disturbed sleep in autism, which could open the door to future treatment that would bring relief to children with autism and their caregivers.

"Poor sleep is not only a problem for individuals with autism but is also one of the top concerns among caregivers," said Lucia Peixoto, an assistant professor in the WSU Elson S. Floyd College of Medicine and the principal investigator and senior author on the study. "In addition, there is a clear link between sleep problems and severity of core autism symptoms such as social and communication challenges and repetitive behaviors. If we can get to the root of these sleep issues, this could potentially help ease other autism symptoms as well."

Published in the open access journaleLife, the team's findings suggest that sleep problems in patients with autism spectrum disorder may be linked to a mutation in the geneSHANK3that in turn regulates the genes of the body's 24-hour day and night cycle. Their study showed that people who were missing theSHANK3gene and mice that lacked part of the gene had difficulty falling asleep. Their study in mice also strongly supported one possible explanation as to why individuals with autism have trouble falling asleep: It's not that they are not sleepy. They are sleepy, but they simply cannot fall asleep.

Peixoto and her colleagues took a multistep approach to their study. First, they analyzed sleep data from patients with Phelan-McDermid syndrome (PMS), a genetic disorder that often goes hand in hand with autism and is thought to be related to theSHANK3gene. They found that PMS patients who are missing theSHANK3gene have trouble falling asleep and wake up multiple times during the night, starting at age five.

"Many kids with Phelan-McDermid syndrome sleep less than six hours a night, and their sleep is really poor across their lifespan so it's an ongoing source of difficulty for them," said postdoctoral research associate and co-first author Hannah Schoch.

Schoch along with co-first author and postdoctoral research associate Ashley Ingiosi did much of the hands-on work for the study. For the next step, Ingiosi studied sleep in a mouse model of PMS, which used mice that are missing a part of theShank3gene and wild-type control mice.

When kept on a light-dark cycle of 12 hours each and allowed to sleep normally, mice with theShank3mutation spent more time awake at the end of the dark period, when nocturnal mice would normally take a nap. The data also suggested that the quality of their deep sleep was reduced.

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In a subsequent sleep deprivation experiment, all mice were kept awake for the first five hours of their main sleep phase.

"What we found is that theShank3mutant mice were able to build up a need for sleep to the same extent as wild-type mice, which tells us that they were sleepy," Ingiosi said. "But it actually took them twice as long to fall asleep."

A gene expression analysis of these mice showed decreased activity in a group of genes related to the body's circadian clock, which helps to maintain a 24-hour day/night rhythm. Sleep deprivation made this worse -- the team found that the number of genes that didn't turn on correctly was twice as high in sleep deprived mutant mice. This suggests that sleep deprivation exaggerates the genetic differences betweenShank3mutants and wild-type mice, Peixoto said, which may translate to a worsening of symptoms in people who have conditions associated withSHANK3mutations, such as PMS and autism.

The final experiment looked at how changes in circadian clock genes affected daily activity patterns inShank3mutant mice. They showed that the decrease in circadian clock gene activity did not change the timing of daily rest-activity rhythms in the mice, but -- surprisingly -- did make them less active.

Follow-up studies to be conducted by the research team will delve deeper into two questions: whether the sleep issues in mice withShank3mutations are present at birth or develop over time and what the mechanism is by whichSHANK3mutations cause sleep issues.

"If we are able to understand the molecular mechanisms underlying the sleep problem inShank3mutant mice, we expect that this will also strongly relate to sleep problems in autism in general," Peixoto said. "And that would suggest novel points of intervention."

Story Source:

Materialsprovided byWashington State University. Original written by Judith Van Dongen.注意:内容可能被编辑风格d length.


Journal Reference:

  1. Ashley M Ingiosi, Hannah Schoch, Taylor Wintler, Kristan G Singletary, Dario Righelli, Leandro G Roser, Elizabeth Medina, Davide Risso, Marcos G Frank, Lucia Peixoto.Shank3 modulates sleep and expression of circadian transcription factors.eLife, 2019; 8 DOI:10.7554/eLife.42819

Cite This Page:

Washington State University. "Gene variant linked to sleep problems in autism." ScienceDaily. ScienceDaily, 29 April 2019. .
Washington State University. (2019, April 29). Gene variant linked to sleep problems in autism.ScienceDaily. Retrieved July 16, 2023 from www.koonmotors.com/releases/2019/04/190429094907.htm
Washington State University. "Gene variant linked to sleep problems in autism." ScienceDaily. www.koonmotors.com/releases/2019/04/190429094907.htm (accessed July 16, 2023).

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